Certificate of Analysis
Human COT1 DNA
Cat No:DNA015 Concentration
2.2 mg/ml

Solution in10mM Tris-HCI, 1 mM EDTA, pH 7.4

 Quality Control

●Average fragments size:50-300 bp;

●A260/A280 ratio:1.6-2.0;

●Amount of genomic (non-repetitive DNA); less than 5%.

The product and the raw material are tested for the absence of HIV1 & 2 RNA, HCV RNA and HBV DNA,using the tagman real-time PCR reagents on Bio Rad CFX 96 instrument. 

Transportation: on blue ice

Storage: at - 20℃ for more than 12 months. We recommend thawing the material slowly at 4℃ before use, 

Description

COT Human DNA is prepared from human placental DNA by shearing, denaturing, and reannealing under conditions that enrich these repetitive elements. The product is prepared from male human placental DNA, exclusively.

The COT I fraction of human genomic DNA consists largely of rapidly annealing repetitive elements. These interspersed repetitive sequences (IRS), such as SINEs (small interspersed repetitive elements, e.g., Alu elements) and LINEs (large interspersed repetitive elements, e.g., L1 elements), are distributed ubiquitously throughout the genome. 

Applications

●In situ suppression(CISS) hybridizations 

●Hybridization to micro arrays

●Other In situ hybridizations

●Filter hybridizations