Table 1. Kit Components and Storage
Material | Amount | Concentration | Storage | Stability |
Qubit ssDNA Assay Kit (Cat No.CMQ005) | ||||
Qubit ssDNA Reagent (Component A) | 200μL | 200X in DMSO | 2-8 ℃ Protect from light | The product is stable for at least 6 months when stored as directed. |
Qubit ssDNA Buffer (Component B) | 50mL | 1X | ||
Qubit ssDNA Standard#1 (Component C) | 200μL | 0 ng/μL in TE buffer | ||
Qubit ssDNA Standard#2 (Component D) | 200μL | 20 ng/μL in TE buffer | ||
Approximate fluorescence excitation/emission maxima, in nm: 500/530, bound to DNA.
Product Description
The Qubit ssDNA Assay Kit provides an easy and accurate quantitation for ssDNA or oligonucleotides. The kit is not selective for ssDNA over dsDNA or RNA, but it will not detect contaminating protein or nucleotides. The assay kit is highly reliable in detecting ssDNA ranging from 1 to 200 ng, and offers advantages in stability, linear dynamic range, and sensitivity over other traditional of DNA quantitation.The kit contains concentrated assay reagent, dilution buffer, and pre-diluted ssDNA standards. The assay is performed at room temperature. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 μl and 50 μl is acceptable), and read the fluorescence using fluorescence plate reader or Fluorometer such as Qubit® or Fluorometer. The kit is well tolerated to common contaminants such as proteins, salts, solvents and detergents.
Handling and Disposal
There is no safety data available for Qubit ssDNA reagent. Treat the Qubit ssDNA reagent with the safety precautions as other potentially harmful reagents and to dispose of the reagent in accordance with local regulations. Centrifuge the Qubit ssDNA reagent and the ssDNA standards before opening vials to minimize loss on the cap. Use properly calibrated pipettes for best accuracy.
