Introduction：
Plasmid Mini Purification Kit is designed to isolate up to 30 µg of high-quality plasmid DNA from 1-6 mL bacterial cultures in less than 30 minutes.
Plasmid DNA purification follows the alkaline-lysis method and is simplified with Spin Column technology into three quick steps: Bind, Wash, and Elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, transformation, PCR and DNA sequencing.
Features:
● Rapid – Purification of plasmid DNA in less than 30 minutes.
● Safe – No Phenol/chloroform extractions.
● High-quality – DNA is suitable for a variety of downstream applications.
Technical Specifications | |
Applications | Plasimd DNA isolation from 1-6 mL bacterial cultures. |
Sample Source | LB culture |
Sample Input | 1-6 mL high-copy plasimd, 10 mL low-copy plasimd |
Elution Volume | 30-100 μL |
Technology | Silica spin column |
Processing Mode | Manual, Centrifugation/Vacuum |
