Prestained Protein marker is a ready-to-use formulation which does not require boiling,Lot-to-lot variation of apparent molecular weight of prestained proteins is less than 3%.

Preparation：

1.Thaw the ladder at room temperature for a few minutes to dissolve precipitated solids.

 Do not boil!

2.Mix gently, but thoroughly, to ensure the solution is homogeneous.

3.Load the following volumes of the ladder on an SDS-polyacrylamide gel:

 - 5 μL per well for mini gel,

 -10 μL per well for large gel.

4.Use the same volumes for Western blotting.

5.The loading volumes listed above are recommended for gels with a thickness of 0.75-1.0 mm. 

 The loading volume should be doubled for 1.5 mm thick gels.